2009•China Environmental ScienceRequires access
Isolation, identification and characteristics of quinone compounds enhancing dye-decolorizing bacterium.
Ling Jiao, LüHong, Jiti Zhou, Cui DeTao, Jing Wang
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Abstract
In the presence of quinone compounds,a strain(designated as JL) with the capacity to decolorize azo dyes was isolated.The strain was identified as Bacillus cereus JL based on morphological,16S rDNA and 16S-23S spacer region sequence analysis.The optimal conditions for the decolorization of Acid Red 3R by strain JL were:1g/L glucose,pH 5～7,30℃,inoculation amount 0.25g/L.Anthraquinone-2-sulfonate(AQS),anthraquinone-2,6-disulfonate(AQDS) and 2-hydroxy-1,4-naphthoquinone(Lawsone) could improve the decolorization rate of Acid Red 3R,and AQS was most effective.Addition of 0.1 mmol/L AQS significantly increased the decolorization rate of 2.0mmol/L Acid Red 3R by strain JL,and the strain could decolorize other six azo dyes.According to the decolorization product of Acid Red 3R identified using HPLC-MS,strain JL could make cleavage for azo bond and AQS only acted as redox mediator in whole process.