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2002•Journal of Veterinary Diagnostic InvestigationRequires access

In Situ Detection of Cellular and Abnormal Isoforms of Prion Protein in Brains of Cattle with Bovine Spongiform Encephalopathy and Sheep with Scrapie by Use of a Histoblot Technique

Kumiko KIMURA, Takashi Yokoyama, Makoto HARITANI, Minoru Narita, Peter Belleby, Julie E. Smith, Y. I. Spencer

Open publisher page 9 citations

Abstract

To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrP(C)) and abnormal (PrP(Sc)) isoforms of the prion protein. In BSE- or scrapie-affected brains, the Prp(C) signal decreased, especially in those areas where the PrP(Sc) signal was detected.

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What this paper is about

To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrP(C)) and abnormal (PrP(Sc)) isoforms of the prion protein. In BSE- or scrapie-affected brains, the Prp(C) signal decreased, especially in those areas where the PrP(Sc) signal was detected.

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Available abstract

To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrP(C)) and abnormal (PrP(Sc)) isoforms of the prion protein. In BSE- or scrapie-affected brains, the Prp(C) signal decreased, especially in those areas where the PrP(Sc) signal was detected.

Key concepts: Scrapie, Bovine spongiform encephalopathy, Prion protein, Gene isoform, Transmissible spongiform encephalopathy, Virology, Biology, Prion Proteins

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In Situ Detection of Cellular and Abnormal Isoforms of Prion Protein in Brains of Cattle with Bovine Spongiform Encephalopathy and Sheep with Scrapie by Use of a Histoblot Technique — Research Paper | ScholarLens