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1965•Canadian Journal of Genetics and CytologyRequires access

A RAPID FEULGEN SQUASH METHOD FOR APHID CHROMOSOMES

M. D. MacDonald, A. M. Harper

Open publisher page 11 citations

Abstract

The cytology and cyto-taxonomy of aphids have been neglected in recent years owing to the lack of a quick, simple technique for preparing specimens. Ris (1942), Shinji (1931), Morgan (1909), and Stevens (1905) used sectioned material in their investigations. Collinp ( 1955), Dionne and Spicer (1957), and Cognetti (1961) used squash techn~ques. The squash method of Dionne and Sp~cer produces good clear preparations but the staining wit11 Gomori's hcmatosylin and the handling of aphids are slow and cumbersome. This paper describes a Feulgen squash method that is rapid, simple, and cff ective.

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What this paper is about

The cytology and cyto-taxonomy of aphids have been neglected in recent years owing to the lack of a quick, simple technique for preparing specimens. Ris (1942), Shinji (1931), Morgan (1909), and Stevens (1905) used sectioned material in their investigations. Collinp ( 1955), Dionne and Spicer (1957), and Cognetti (1961) used squash techn~ques. The squash method of Dionne and Sp~cer produces good clear preparations but the staining wit11 Gomori's hcmatosylin and the handling of aphids are slow and cumbersome. This paper describes a Feulgen squash method that is rapid, simple, and cff ective.

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OpenAlex reports 11 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

The cytology and cyto-taxonomy of aphids have been neglected in recent years owing to the lack of a quick, simple technique for preparing specimens. Ris (1942), Shinji (1931), Morgan (1909), and Stevens (1905) used sectioned material in their investigations. Collinp ( 1955), Dionne and Spicer (1957), and Cognetti (1961) used squash techn~ques. The squash method of Dionne and Sp~cer produces good clear preparations but the staining wit11 Gomori's hcmatosylin and the handling of aphids are slow and cumbersome. This paper describes a Feulgen squash method that is rapid, simple, and cff ective.

Key concepts: Squash, Biology, Feulgen stain, Aphid, Botany, Genetics, DNA

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