Effects of propofol pretreatment on the AQP-1 expression after HK-2 cells injured by anoxia-reoxygenation.

Objective To determine what effects propofol exerts on aqp-1 mRNA expression after HK-2 cells injured by anoxiareoxygenation.Methods HK-2 cells were randomly assigned to one of 4 groups:Control group(group A),CoCl2 group(group B).300 μmol/L CoCl2 were added to the cultured HK-2 cells,and then maintained with no serum media for 24 h.In intralipid group(group C),HK-2 cells were pretreated with 10% intralipid 90 μl 1h and treated as group B.In propofol group(group D)HK-2 cells were pretreated with 25 μmol/L propofol for 1 hour and treated as group B.MTT method was employed to detect the proliferation of HK-2 cells and RT-PCR to show AQP-1 mRNA and bcl-2 mRNA expression.Results After pretreated with 25 μmol/L propofol,the proliferation of HK-2 cells was increased(P 0.01).The expression of AQP-1 gene was significantly down-regulated and bcl-2 gene significantly up-regulated(P 0.01).Conclusion Pretreatment with 25 μmol/L propofol can protect HK-2 cells against anoxia-reoxygenation injury by regulating the expression of AQP-1 mRNA and bcl-2 mRNA.