1986•Proceedings of the National Academy of SciencesOpen access

Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity.

Edward B. Leof, Jacqueline A. Proper, Anton Scott Goustin, Gary D. Shipley, Paul E. DiCorleto, Harold L. Moses

Abstract

Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth factor beta resulted in an early induction of c-sis mRNA. The increase in c-sis mRNA was followed by a corresponding increase in protein similar to platelet-derived growth factor (PDGF) in the culture medium. In addition, PDGF-regulated genes (c-fos and c-myc) were stimulated by transforming growth factor beta with delayed kinetics relative to that seen in other cell systems with direct PDGF stimulation. A model is proposed in which the monolayer mitogenicity of transforming growth factor beta is mediated by the induction of c-sis and PDGF and the subsequent autocrine stimulation of c-fos, c-myc, and other PDGF-inducible genes.

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Key concepts: Autocrine signalling, Platelet-derived growth factor receptor, Platelet-derived growth factor, Growth factor, Biology, Transforming growth factor, Transforming growth factor beta, Transforming growth factor, beta 3