Factors affecting extracellular and intracellular polysaccharide production in submerged cultivation of Tricholoma Mongolicum

The mycelia of Tricholoma Mongolicum M-1 was used to study the effects of various carbon and nitrogen sources, initial pH, temperature, inoculums size, rotation speed and medium capacity on the production of extracellular polysaccharide (EPS) and intracellular polysaccharide (IPS) in shake-flask cultures. The optimization of submerged culture conditions were studied using single factor test and orthogonal matrix method. The results showed that the optimal medium contained 30 g/L yeast extract, 20 g/L glucose, 0.3% KH2PO4 and 0.3% MgSO4, then the optimal cultivation conditions were: pH 6.5, temperature 25°C, inoculums size 12% and rotation speed 180 rpm. Under these optimal conditions, the maximum EPS and IPS concentrations reached 1.187 and 1.073 g/L respectively, which were twice as much as the yields at the basal medium.   Key words: Tricholoma Mongolicum, extracellular polysaccharide, intracellular polysaccharide.