Alkaloid constitution of Nerium oleander using gas chromatography-mass spectroscopy (GC-MS)

Oleander is an evergreen shrub or small tree from 5 to 25-ft tall containing gummy sticky sap in the dogbane family Apocynaceae. In this study, the alkaloid compounds of Nerium oleander (NO) have been evaluated. The chemical compositions of the leaf ethanol extract of N. oleander were investigated using gas chromatography-mass specroscopy (GC-MS). GC-MS analysis of N. oleander alkaloid leaf ethanol extract revealed the existence of the 5-hydrooxy methylfurfural, β-d-allopyranoside, methyle 6-dioxy-2-o methyl, cycloheptasiloxane, tetradecamethyle, cyclooctasiloxane, hexadecamethyl, cyclononasiloxane, octadecamethyl, cyclodecasiloxane, eicosamethyl, 2-cyclopenten-1-one, 2-hydroxy-3-methyl, 9.12.15-octadecatrienoic acid, 2,3bis[trimethylsilyl]oxy] propyl ester, octadecane, 3-ethyl-5-(2-ethylbutyl), 1-monolinoleoylglycerol trimethylsilyl ether, 1.1.3.3.5.5.7.7.9.9-decamethyl-9-(2-methyl propoxy)pentasiloxane, 2-cyclohexen-1-one, 4-(hydroxybutyle)-3.5.5-trimethyl, octasiloxane, 1.1.3.3.5.5.7.7.9.9.11.11.13.13.15.15-hexadecamethyl and 3-eicosene. Eight chemical alkaloids constituents have been identified from methanolic extract of the Nerium oleander. Key words: Alkaloids, methanol, gas chromatography-mass spectroscopy (GC-MS) analysis, Nerium oleander.