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1983•Journal of General VirologyRequires access

Affinity Chromatography of Human Fibroblast Interferon (IFN-beta1) by Monoclonal Antibody Columns

Daniela Novick, Zelig Eshhar, Orit Gigi, Zipporah Marks, Michel Revel, Menachem Rubinstein

Open publisher page 37 citations

Abstract

Eight hybridomas secreting monoclonal antibodies specific to human fibroblast interferon (IFN-beta 1) were identified by a new screening procedure based on immune precipitation with a second antibody and recovery of interferon activity from the immune precipitate. Immunoadsorbents were prepared from these monoclonal antibodies and used for purification of IFN-beta 1. Electrophoretically pure IFN-beta 1, having a specific activity of 3 X 10(8) to 6 X 10(8) units/mg was obtained by a single passage of partially purified IFN-beta 1 on these immunoadsorbents.

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What this paper is about

Eight hybridomas secreting monoclonal antibodies specific to human fibroblast interferon (IFN-beta 1) were identified by a new screening procedure based on immune precipitation with a second antibody and recovery of interferon activity from the immune precipitate. Immunoadsorbents were prepared from these monoclonal antibodies and used for purification of IFN-beta 1. Electrophoretically pure IFN-beta 1, having a specific activity of 3 X 10(8) to 6 X 10(8) units/mg was obtained by a single passage of partially purified IFN-beta 1 on these immunoadsorbents.

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OpenAlex reports 37 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Eight hybridomas secreting monoclonal antibodies specific to human fibroblast interferon (IFN-beta 1) were identified by a new screening procedure based on immune precipitation with a second antibody and recovery of interferon activity from the immune precipitate. Immunoadsorbents were prepared from these monoclonal antibodies and used for purification of IFN-beta 1. Electrophoretically pure IFN-beta 1, having a specific activity of 3 X 10(8) to 6 X 10(8) units/mg was obtained by a single passage of partially purified IFN-beta 1 on these immunoadsorbents.

Key concepts: Monoclonal antibody, Interferon, Biology, Antibody, Affinity chromatography, Fibroblast, Molecular biology, Virology

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Affinity Chromatography of Human Fibroblast Interferon (IFN-beta1) by Monoclonal Antibody Columns — Research Paper | ScholarLens