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2003•Journal of Clinical MicrobiologyOpen access

Evaluation of Acridine Orange Staining as a Replacement of Subcultures for BacT/ALERT-Positive, Gram Stain-Negative Blood Cultures

Hildegard Adler, Nathalie Baumlin, Reno Frei

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Abstract

Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.

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Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.

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Available abstract

Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.

Key concepts: Acridine orange, Gram staining, Staining, Stain, Acridine, Microbiology, Biology, Orange (colour)

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