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1991•Journal of Veterinary Medical ScienceOpen access

Partial restriction map of salmonid herpesvirus(Oncorhynchus masou virus)DNA for three restriction enzymes BamHI, EcoRI and XhoI.

Deng‐Fu Guo, Hiroshi Kodama, Misao Onuma

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Abstract

Cleavage of Oncorhynchus masou virus (OMV) DNA with restriction endonucleases BamHI, EcoRI, and XhoI resulted in 28, 26 and 17 fragments, respectively. Based on the molecular weights of digested fragments and those molar ratio. OMV DNA showed the molecular weight of about 100 x 10(6). Twenty out of 28 BamHI fragments of OMV DNA were successfully cloned into pBR322 vector. Restriction map of OMV DNA was constructed by blotting hybridization and double-digestion. The data suggested that terminal repeat of the end fragments of OMV DNA molecule was not existence.

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Cleavage of Oncorhynchus masou virus (OMV) DNA with restriction endonucleases BamHI, EcoRI, and XhoI resulted in 28, 26 and 17 fragments, respectively. Based on the molecular weights of digested fragments and those molar ratio. OMV DNA showed the molecular weight of about 100 x 10(6). Twenty out of 28 BamHI fragments of OMV DNA were successfully cloned into pBR322 vector. Restriction map of OMV DNA was constructed by blotting hybridization and double-digestion. The data suggested that terminal repeat of the end fragments of OMV DNA molecule was not existence.

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Available abstract

Cleavage of Oncorhynchus masou virus (OMV) DNA with restriction endonucleases BamHI, EcoRI, and XhoI resulted in 28, 26 and 17 fragments, respectively. Based on the molecular weights of digested fragments and those molar ratio. OMV DNA showed the molecular weight of about 100 x 10(6). Twenty out of 28 BamHI fragments of OMV DNA were successfully cloned into pBR322 vector. Restriction map of OMV DNA was constructed by blotting hybridization and double-digestion. The data suggested that terminal repeat of the end fragments of OMV DNA molecule was not existence.

Key concepts: XhoI, EcoRI, BamHI, Restriction enzyme, Molecular biology, Biology, Southern blot, Restriction map

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Partial restriction map of salmonid herpesvirus(Oncorhynchus masou virus)DNA for three restriction enzymes BamHI, EcoRI and XhoI. — Research Paper | ScholarLens