1997•Angewandte Chemie International Edition in EnglishOpen access

Creation of Enantioselective Biocatalysts for Organic Chemistry by In Vitro Evolution

Manfred T. Reetz, Albin Zonta, Klaus Schimossek, Karl‐Erich Jaeger, Klaus Liebeton

Abstract

Evolution in the test tube: With the help of the error-prone polymerase chain reaction as a method for random mutagenesis, an efficient gene-expression system, and a screening test for the rapid identification of enantioselective catalysts, it is possible to increase sequentially the ee value of an unselective lipase-catalyzed ester hydrolysis (see picture on the right).

Open-access reader

About this research paper

What this paper is about

Why it matters

OpenAlex reports 381 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Enantioselective synthesis, Lipase, Hydrolysis, Chemistry, Mutagenesis, Directed evolution, Catalysis, Pancreatic lipase