1975Canadian Journal of MicrobiologyRequires access

Comparison of several methods for the isolation of salmonellae from egg products

M. van Schothorst, Frans M. van Leusden

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Abstract

Lactose broth and buffered peptone water proved to be equally effective as preenrichment media in the isolation of salmonellae from egg products. With tetrathionate bile brilliant green broth, incubated at 43 degrees C as enrichment medium, more samples were found to be positive than with selenite cystine broth incubated at 37 degrees C or 43 degreesC and selenite brilliant green broth incubated at 37 degrees C. As selective agar, brilliant green agar proved to be more reliable than bismuth sulfite agar. These results indicate that the ISO method for the isolation of salmonellae from meat and meat products can be successfully used for the examination of egg products.

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What this paper is about

Lactose broth and buffered peptone water proved to be equally effective as preenrichment media in the isolation of salmonellae from egg products. With tetrathionate bile brilliant green broth, incubated at 43 degrees C as enrichment medium, more samples were found to be positive than with selenite cystine broth incubated at 37 degrees C or 43 degreesC and selenite brilliant green broth incubated at 37 degrees C. As selective agar, brilliant green agar proved to be more reliable than bismuth sulfite agar. These results indicate that the ISO method for the isolation of salmonellae from meat and meat products can be successfully used for the examination of egg products.

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Available abstract

Lactose broth and buffered peptone water proved to be equally effective as preenrichment media in the isolation of salmonellae from egg products. With tetrathionate bile brilliant green broth, incubated at 43 degrees C as enrichment medium, more samples were found to be positive than with selenite cystine broth incubated at 37 degrees C or 43 degreesC and selenite brilliant green broth incubated at 37 degrees C. As selective agar, brilliant green agar proved to be more reliable than bismuth sulfite agar. These results indicate that the ISO method for the isolation of salmonellae from meat and meat products can be successfully used for the examination of egg products.

Key concepts: Isolation (microbiology), Biology, Microbiology, Biotechnology, Food science

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