2009•Journal of PhytopathologyRequires access

Immunodiagnosis of Parietaria mottle virus in Tomato Crops Using a Polyclonal Antiserum against its Coat Protein Expressed in a Bacterial System

Frederic Aparicio, J. Aramburu, Salvador Soler, Luis Galipienso, Fernando Nuez, Vicente Pallás, Carmelo López

Abstract

Abstract Recombinant DNA technology was used to raise a polyclonal antiserum against the coat protein (CP) of Parietaria mottle virus (PMoV). The CP gene was expressed in Escherichia coli as a fusion to a 6xHis tag and purified by affinity chromatography. Recombinant purified protein was used as antigen to raise a polyclonal antiserum. This polyclonal antiserum consistently detected PMoV specifically infected tomato plants from different commercial tomato crops by indirect enzyme‐linked immunosorbent assay (I‐ELISA) and direct tissue‐printing immunoassay (DTBIA).

About this research paper

What this paper is about

Why it matters

OpenAlex reports 7 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Polyclonal antibodies, Antiserum, Biology, Recombinant DNA, Fusion protein, Immunoassay, Affinity chromatography, Escherichia coli