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2013•Journal of Plankton ResearchOpen access

A DNA-based method for investigating feeding by copepod nauplii

Carrie Craig, Wim Kimmerer, Sarah Cohen

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Abstract

We developed an approach to recover prey DNA from gut contents of nauplii of a predatory copepod by blocking the detection of predator with ligase and a blocking oligonucleotide and amplifying prey DNA with universal primers. Adding 25 U of ligase improved predator clamping, while 100 and 200 U reduced efficiency. Field-collected nauplii consumed a broad range of prey. The method paves the way to investigations of the diets of small predators at all life stages.

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What this paper is about

We developed an approach to recover prey DNA from gut contents of nauplii of a predatory copepod by blocking the detection of predator with ligase and a blocking oligonucleotide and amplifying prey DNA with universal primers. Adding 25 U of ligase improved predator clamping, while 100 and 200 U reduced efficiency. Field-collected nauplii consumed a broad range of prey. The method paves the way to investigations of the diets of small predators at all life stages.

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Available abstract

We developed an approach to recover prey DNA from gut contents of nauplii of a predatory copepod by blocking the detection of predator with ligase and a blocking oligonucleotide and amplifying prey DNA with universal primers. Adding 25 U of ligase improved predator clamping, while 100 and 200 U reduced efficiency. Field-collected nauplii consumed a broad range of prey. The method paves the way to investigations of the diets of small predators at all life stages.

Key concepts: Copepod, Predation, Biology, Predator, Zoology, Ecology, Crustacean

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