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2011•Current Protocols in Mouse BiologyRequires access

One‐Step Vital Staining of Presynaptic Terminals and Post‐Synaptic Receptors at Neuromuscular Junctions in Mouse Skeletal Muscle

Richard R. Ribchester

Open publisher page 2 citations

Abstract

This protocol describes vital staining of neuromuscular junctions in the mouse triangularis sterni muscle in one incubation step, combining presynaptic, motor nerve terminal staining with the styryl dye FM1-43, which labels recycling synaptic vesicles, and TRITC-α-bungarotoxin, which labels acetylcholine receptors in the motor endplate membrane. Curr. Protoc. Mouse Biol. 1:489-496 © 2011 by John Wiley & Sons, Inc.

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What this paper is about

This protocol describes vital staining of neuromuscular junctions in the mouse triangularis sterni muscle in one incubation step, combining presynaptic, motor nerve terminal staining with the styryl dye FM1-43, which labels recycling synaptic vesicles, and TRITC-α-bungarotoxin, which labels acetylcholine receptors in the motor endplate membrane. Curr. Protoc. Mouse Biol. 1:489-496 © 2011 by John Wiley & Sons, Inc.

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Available abstract

This protocol describes vital staining of neuromuscular junctions in the mouse triangularis sterni muscle in one incubation step, combining presynaptic, motor nerve terminal staining with the styryl dye FM1-43, which labels recycling synaptic vesicles, and TRITC-α-bungarotoxin, which labels acetylcholine receptors in the motor endplate membrane. Curr. Protoc. Mouse Biol. 1:489-496 © 2011 by John Wiley & Sons, Inc.

Key concepts: Neuromuscular junction, Acetylcholine receptor, Motor Endplate, Motor nerve, Synaptic vesicle, Acetylcholine, Biology, Neuroscience

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One‐Step Vital Staining of Presynaptic Terminals and Post‐Synaptic Receptors at Neuromuscular Junctions in Mouse Skeletal Muscle — Research Paper | ScholarLens