82 INTESTINAL REVERSAL LOOP (RL) IN GROWING RATS. A MODEL OF INTESTINAL DYSMOTRICITY AND BACTERIAL TRANSLOCATION.

In human, a surgical procedure of a small bowel RL in continuity has been proposed for very short bowel syndrome to increase intestinal absorption and transit time. This technic however could exposed to bacterial translocation and bacteriemia originated from the gut. The aim of our study was to analyse in growing rats (125gr) n=12 the potentila role of a 10cm RL anastomosed in continuity with the remaining small bowel on small intestine flora and mesenteric lymph nodes (MLN) spleen (S) and liver (L) translocation, and compared to a control group C of 7 rats matched for age and weight. At day 8 post surgery the RL group was sacrified and the MLN, L and S were aseptically collected as well as a 10cm small intestine segment above and below the RL. Bacterial culture of samples homogenized and diluted in sterile saline were performed on different selective media under aerobic and anaerobic conditions. Colony counting were performed using the Spiral systems. Results were expressed in Log10 of colony forming units (CFU)/g of wet tissues. A significant bacterial owergrowth was observed in the jejunum and the ileum of the RL group as compared to C. Table No difference was observed for anaerobes. A significant bacterial translocation in S was observed in RL/C. In conclusion the model of small intestine RL in growing rats is appropriate to study intestinal dysmotricity, bacterial overgrowth and translocation.