2003•Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIERequires access

Fluorescence techniques in biomedical diagnostics

Herbert Schneckenburger, Reinhard Sailer, Karl Stock, Wolfgang S. L. Strauß, R. Steiner

Abstract

An overview on some advanced techniques of fluorescence spectroscopy and fluorescence microscopy is given. These techniques include time-resolved fluorescence spectroscopy, energy transfer spectroscopy (FRET), total internal reflection fluorescence microscopy (TIRFM) and fluorescence lifetime imaging (FLIM). The principle of these methods is explained, and numerous applications are described.

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Available abstract

Key concepts: Förster resonance energy transfer, Fluorescence, Microscopy, Total internal reflection fluorescence microscope, Spectroscopy, Fluorescence spectroscopy, Fluorescence-lifetime imaging microscopy, Fluorescence microscope