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2005•Journal of Liquid Chromatography & Related TechnologiesRequires access

HPLC Method for the Pharmacokinetics Study of Acemetacin in Human Plasma

Eunmi Ban, Jung‐Hye Cho, Dong‐Jin Jang, Xiang‐Lan Piao, Jin‐Ki Kim, Jun‐Pil Ji, Chong‐Kook Kim

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Abstract

A reproducible and specific high performance liquid chromatography (HPLC) method was developed to quantity plasma levels of acemetacin and its metabolite, indometacin in order to conduct a pharmacokinetics study. Plasma samples were prepared by liquid‐liquid extraction using ethyl acetate. Extracted samples were analyzed on a reverse‐phase C18 column with a mobile phase consisting of 20 mM phosphate buffer (pH 2.9) and acetonitrile (60:40, v/v) and UV detection at 254 nm. Using this method, the pharmacokinetics study was carried out on eight volunteers after a single oral administration.

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What this paper is about

A reproducible and specific high performance liquid chromatography (HPLC) method was developed to quantity plasma levels of acemetacin and its metabolite, indometacin in order to conduct a pharmacokinetics study. Plasma samples were prepared by liquid‐liquid extraction using ethyl acetate. Extracted samples were analyzed on a reverse‐phase C18 column with a mobile phase consisting of 20 mM phosphate buffer (pH 2.9) and acetonitrile (60:40, v/v) and UV detection at 254 nm. Using this method, the pharmacokinetics study was carried out on eight volunteers after a single oral administration.

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OpenAlex reports 6 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

A reproducible and specific high performance liquid chromatography (HPLC) method was developed to quantity plasma levels of acemetacin and its metabolite, indometacin in order to conduct a pharmacokinetics study. Plasma samples were prepared by liquid‐liquid extraction using ethyl acetate. Extracted samples were analyzed on a reverse‐phase C18 column with a mobile phase consisting of 20 mM phosphate buffer (pH 2.9) and acetonitrile (60:40, v/v) and UV detection at 254 nm. Using this method, the pharmacokinetics study was carried out on eight volunteers after a single oral administration.

Key concepts: Chemistry, Pharmacokinetics, Chromatography, Human plasma, High-performance liquid chromatography, Plasma, Pharmacology, Physics

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