An electrophoretic comparison of the enzymes of Saccharomyces yeasts.

The relationships among 36 strains of 17 Saccharomyces species were studied by comparing ten enzymes electrophoretically. Twelve of the 13 strains of Saccharomyces cerevisiae tested produced rather similar patterns, but one distiller's yeast previously classified as Saccharomyces formosensis differed in fructose-1, 6-bisphosphate aldolase (EC 4.1.2.13). Three Saccharomyces uvarum strains split into two categories with quite different enzyme patterns. One included strains previously named Saccharomyces carlsbergensis and was similar to S. cerevisiae in itsenzyme patterns. The electrophoretic patterns of S. cerevisiae, S. bayanus, S. chevalieri, S. diastaticus, and S. italicus were nearly identical. Strains of Saccharomyces delbrueckii, S. fermentati, S. rosei, and S. saitoanus, belonging to the so-called Torulaspora group, had similar electrophoretic patterns which were readily distinguishable from those of S. cerevisiae. The patterns of Saccharomyces bailii, S. bisporus, S. florentinus, and S. rouxii, belonging to the so-called Zygosaccharomyces group, differed from each other and from the S. cerevisiae and Torulaspora strains.