2002•Physiologia PlantarumRequires access
Maize replicative α‐type DNA polymerase: separation of polymerase and primase activities and recognition of primase subunits
Elpidio García, Patricia Laquel, Michel Castroviejo, Javier Plasencia, Jorge M. Vázquez‐Ramos
Open publisher page 5 citations
Abstract
DNA polymerase and DNA primase activities in the maize alpha-type DNA polymerase 2 were dissociated and DNA polymerase-free DNA primase was studied. DNA primase synthesized primers that were 8-34 nucleotides long, with more intense bands at 15-17 nucleotides in length. DNA polymerase 1 (a putative delta-type enzyme) or DNA polymerase 2 were assayed after template-priming with purified DNA primase and showed a differential use of templates: whereas DNA polymerase 2 used a polydT template more efficiently than a natural template, DNA polymerase 1 used both of them poorly. The molecular size of DNA primase was estimated to be 68 kDa by gel filtration, western blotting and by a DNA primase 'trapping' assay.