2011•Scandinavian Journal of Infectious DiseasesRequires access

Imported brucellosis in Denmark: Molecular identification and multiple-locus variable number tandem repeat analysis (MLVA) genotyping of the bacteria

Huma Aftab, Rimtas Dargis, Jens Jørgen Christensen, Philippe Le Flèche, Michael Kemp

Abstract

A polymerase chain reaction was used to identify Brucella species isolated from humans in Denmark. Consecutive analysis of referred bacteria and re-examination of historical isolates identified all as Brucella melitensis. Multiple-locus variable number tandem repeat analysis (MLVA) placed the isolates in the previously defined 'East Mediterranean' B. melitensis group.

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Key concepts: Multiple Loci VNTR Analysis, Variable number tandem repeat, Brucella, Brucella melitensis, Genotyping, Brucellosis, Tandem repeat, Biology