Optimization of Chelation of Whey Protein Hydrolysate with Calcium Using Response Surface Method

To optimum the chelation technology of whey protein hydrolysate with calcium, the response surface method was used to investigate optimized technological conditions. The result showed that the optimum process parameters for the whey protein hydrolysate-calcium chelation were whey protein peptide and CaCl2 ratio of 24 : 1 (w/w), whey protein hydrolysate concentration 3.5 %, pH 7.5, reaction time 20 min, reaction temperature 30 °C. Finally, the optimum level was established and whey protein hydrolysate-calcium chelate was obtained, which can provide basic theories for the following function and activity evaluation of this potential calcium supplement.