Rotating frame nuclear overhauser effect: a practical tool for the 1H NMR study of peptides in solution

A new 2D NMR experiment, the rotating frame nuclear Overhauser enhancement (nOe) spectroscopy (ROESY) was recently proposed as an alternative to NOESY for the study of intermediate molecules. Its practical use is here tested on bacillomycin D, a bacterial cyclic lipopeptide. This sequential resonance assignment, based on nOe connectivities at room temperature, shows that the high viscosity which is generally required for conventional nOe measurement is no longer a restriction for choosing a suitable solvent. In comparison to low‐temperature NOESY, short internuclear connectivities can be safely demonstrated because of no spin diffusion, for sequential assignment ofpeptide resonances.