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2006•Molecular Ecology NotesOpen access

Microsatellite markers for Fragaria from ‘Strawberry Festival’ expressed sequence tags

Nahla Victor Bassil, Melissa R. Gunn, Kevin M. Folta, Kim S. Lewers

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Abstract

Abstract We present 37 microsatellite primer pairs developed from a cDNA library of Fragaria xananassa Duch. cv. Strawberry Festival. Polymorphism was high and the number of presumptive alleles of 13 expressed sequence tag–simple sequence repeats (EST–SSRs) in 70 strawberry cultivars ranged from five to 32 per primer pairs, averaging 16.1. Cross‐species amplification was also high and ranged from 89% in Fragaria vesca L. to 100% in the progenitor species of octoploid strawberry, Fragaria chiloensis (L.) Duch. and Fragaria virginiana Duch.

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Abstract We present 37 microsatellite primer pairs developed from a cDNA library of Fragaria xananassa Duch. cv. Strawberry Festival. Polymorphism was high and the number of presumptive alleles of 13 expressed sequence tag–simple sequence repeats (EST–SSRs) in 70 strawberry cultivars ranged from five to 32 per primer pairs, averaging 16.1. Cross‐species amplification was also high and ranged from 89% in Fragaria vesca L. to 100% in the progenitor species of octoploid strawberry, Fragaria chiloensis (L.) Duch. and Fragaria virginiana Duch.

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Available abstract

Abstract We present 37 microsatellite primer pairs developed from a cDNA library of Fragaria xananassa Duch. cv. Strawberry Festival. Polymorphism was high and the number of presumptive alleles of 13 expressed sequence tag–simple sequence repeats (EST–SSRs) in 70 strawberry cultivars ranged from five to 32 per primer pairs, averaging 16.1. Cross‐species amplification was also high and ranged from 89% in Fragaria vesca L. to 100% in the progenitor species of octoploid strawberry, Fragaria chiloensis (L.) Duch. and Fragaria virginiana Duch.

Key concepts: Fragaria, Biology, Microsatellite, Primer (cosmetics), Expressed sequence tag, Botany, Allele, Complementary DNA

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