SIMULTANEOUS QUANTITATIVE DETERMINATION OF SEVEN POLYPHENOL CONSTITUENTS FROM HERBA LOPHATHERI BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

The goal of this research was to develop a simple, rapid, and sensitive method for the simultaneous quantitative determination of trans-p-coumaric acid, isoorientin, orientin, swertiajaponin, vitexin, isovitexin, and luteolin-7-O-β-D-glucoside in Herba Lophatheri by high performance liquid chromatographic (HPLC) with photodiode array detection (PAD). The chromatographic separation was performed on a C18 column (4.6 mm × 250 mm, 5 µm), with a gradient elution program using the mixture of acetonitrile and 1.0% acetic acid (v/v) as mobile phase. The seven components of the standards achieved baseline separation. Regression analysis revealed a linear relationship between the contents and the peak areas of the mixed standard substances. The average recovery rates were 98.11%∼100.23%, and RSD values were less than 5.0%. In summary, a reliable HPLC method for simultaneous determination of the seven compounds in Herba Lophatheri was developed, and provided a scientific basis for the quality control of herbal medicine.