Potential mycotoxin productivity of Alternaria alternata isolated from garden trees

Potential mycotoxin productivity of Alternaria alternata isolated from leaves of Mube (Stauntonia hexaphylla), Hanamizuki (Cornus florida), and Kobushi (Magnolia praecocissima) was investigated with cultures growing on rice medium.By thin-layer chromatography and high-performance liquid chromatography, the presence of alternariol (AOH), alternariol monomethyl ether (AME), and altenuene (ALT) was confirmed in the rice culture extract. A. alternata isolate from Mube leaves produced AOH, AME, and ALT at concentrations of 22.99 mg/kg, 9.13 mg/kg, and 2.53 mg/kg, respectively.A. alternata isolate from Hanamizuki leaves produced AOH, AME, and ALT at concentrations of 1.50 mg/kg, 0.59 mg/kg, and 3.42 mg/kg, respectively.A. alternata isolate from Kobushi leaves produced AOH, AME, and ALT at concentrations of 20.37 mg/kg, 0.95 mg/kg, and 7.25 mg/kg, respectively.These results suggested that A. alternata on garden trees contaminate food and feed with the mycotoxins.