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1975•Acta PaediatricaRequires access

BIOCHEMICAL DIAGNOSIS OF CYSTINOSIS USING LEUCOCYTES

Paul A. Willcox, A. DESMOND PATRICK

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Abstract

Abstract. Willcox, P. and Patrick, A. D. (Institute of Child Health, London, England). Biochemical Diagnosis of Cystinosis using leucocytes. Acta Paediatr Scand 64:132, 1975. –A simple method for the biochemical diagnosis of cystinosis involves incubating leucocytes from a small volume of blood with 35S‐cystine. Non‐protein 35S‐IabeIled compounds are then extracted from the leucocytes and separated by thin‐layer chromatography. The excessive incorporation of 35S‐cystine into the increased cystine pool of cystine cells is easily detected in autoradiography of the chromatograms.

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What this paper is about

Abstract. Willcox, P. and Patrick, A. D. (Institute of Child Health, London, England). Biochemical Diagnosis of Cystinosis using leucocytes. Acta Paediatr Scand 64:132, 1975. –A simple method for the biochemical diagnosis of cystinosis involves incubating leucocytes from a small volume of blood with 35S‐cystine. Non‐protein 35S‐IabeIled compounds are then extracted from the leucocytes and separated by thin‐layer chromatography. The excessive incorporation of 35S‐cystine into the increased cystine pool of cystine cells is easily detected in autoradiography of the chromatograms.

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Available abstract

Abstract. Willcox, P. and Patrick, A. D. (Institute of Child Health, London, England). Biochemical Diagnosis of Cystinosis using leucocytes. Acta Paediatr Scand 64:132, 1975. –A simple method for the biochemical diagnosis of cystinosis involves incubating leucocytes from a small volume of blood with 35S‐cystine. Non‐protein 35S‐IabeIled compounds are then extracted from the leucocytes and separated by thin‐layer chromatography. The excessive incorporation of 35S‐cystine into the increased cystine pool of cystine cells is easily detected in autoradiography of the chromatograms.

Key concepts: Cystinosis, Medicine, Pediatrics, Cystine, Biochemistry, Enzyme, Cysteine, Chemistry

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