2002•Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)Open access
Evaluation of Selective Plating Media for the Detection of Heat- or Freeze-injured Staphylococcus aureus.
Yoichi Onoue, Rie SHINDO, Hiroshi Teranishi, Ichiro Furukawa, Yukie Hasegawa, Tsutomu Maruyama
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Abstract
The efficacy of Baird-Parker (BP) agar, mannitol-salt-egg yolk (MSEY) agar and mannitol salt (MS) agar in detecting Staphylococcus aureus FRI-100 heated at 52 degrees C for 20 min in 100 mmol/L potassium phosphate buffer was determined. Brain heart infusion agar with 1% pyruvate (BHIP agar) supported the highest recovery of injured cells and was used as the control medium. Of the three selective media, significantly higher recovery of heat-injured cells was observed on BP agar than MSEY agar, and the poorest recovery was observed on MS agar (p < 0.05). Low recovery of unheated cells was obtained for MS compared with other media (p < 0.05). A reduction in populations occurred gradually in reagent-grade water stored for 14 days at -20 degrees C. There was no significant difference between BHIP agar and MS agar in the number of freeze-injured cells recovered from 1 to 14 days.
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