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Application of Alfalfa Protoplast Cultures in Genetic Studies

Georgina Kosturkova

Open publisher page 2 citations

Abstract

Some of the applications of Medicago protoplast cultures in mutagenesis, selection and genetic transformation studies are demonstrated. The establishment of reliable protoplast systems with high viability, cell division and regeneration capacity is a prerequisite for successful investigations. When different kinds of treatment were applied, it was important to compromise between the most effective parameters of treatment, protoplast viability and expected genetic changes.

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What this paper is about

Some of the applications of Medicago protoplast cultures in mutagenesis, selection and genetic transformation studies are demonstrated. The establishment of reliable protoplast systems with high viability, cell division and regeneration capacity is a prerequisite for successful investigations. When different kinds of treatment were applied, it was important to compromise between the most effective parameters of treatment, protoplast viability and expected genetic changes.

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Available abstract

Some of the applications of Medicago protoplast cultures in mutagenesis, selection and genetic transformation studies are demonstrated. The establishment of reliable protoplast systems with high viability, cell division and regeneration capacity is a prerequisite for successful investigations. When different kinds of treatment were applied, it was important to compromise between the most effective parameters of treatment, protoplast viability and expected genetic changes.

Key concepts: Protoplast, Biology, Transformation (genetics), Medicago sativa, Selection (genetic algorithm), Mutagenesis, Regeneration (biology), Biotechnology

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