Interaction of human minichromosome maintenance protein‐binding protein with minichromosome maintenance 2–7

It has been reported that minichromosome maintenance protein‐binding protein (MCM‐BP) functions in the formation of the pre‐replication complex, unloading of minichromosome maintenance (MCM)2–7 from chromatin in late S phase, and formation of the cohesion complex by interacting with MCM3–7 proteins, suggesting that MCM‐BP functions in several different reactions during the cell cycle. Here, we examined the interaction of human MCM‐BP with MCM2–7 and structural maintenance of chromosome 3 in synchronized HeLa cells by immunoprecipitation. The results show that MCM‐BP mainly interacts with MCM7 in the Triton‐soluble fraction from S phase and G2 phase cells, and it also interacts with structural maintenance of chromosome 3 in the fraction from G2 phase cells. In vitro studies show that MCM‐BP disassembles MCM2–7 bound to DNA with a fork‐like structure by interacting with MCM3, MCM5, and MCM7. These results suggest that MCM‐BP functions in disassembling MCM2–7 on chromatin during S phase and G2 phase by interacting with MCM3, MCM5, and MCM7. Structured digital abstract MCM-BP physically interacts with MCM6 by anti bait coimmunoprecipitation ( 1 , 2 ) MCM-BP physically interacts with MCM8 by anti bait coimmunoprecipitation ( View interaction ) MCM-BP physically interacts with MCM7 by anti bait coimmunoprecipitation ( View interaction ) MCM-BP physically interacts with MCM2 by anti bait coimmunoprecipitation ( View interaction ) MCM-BP physically interacts with MCM7 , MCM4 , MCM3 , MCM5 and MCM6 by anti tag coimmunoprecipitation ( View interaction ) MCM4 physically interacts with MCM7 by anti bait coimmunoprecipitation ( View interaction ) MCM-BP physically interacts with CDC7 by anti bait coimmunoprecipitation ( View interaction ) MCM-BP physically interacts with PSF2 by anti bait coimmunoprecipitation ( View interaction )