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2011•Journal of BacteriologyOpen access

Sequential Closure of the Cytoplasm and Then the Periplasm during Cell Division in Escherichia coli

Karl Skoog, Bill Söderström, Jerker Widengren, Gunnar von HEIJNE, Daniel O. Daley

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Abstract

To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.

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To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.

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Available abstract

To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.

Key concepts: Periplasmic space, Cytoplasm, Biology, Escherichia coli, Cell division, Fluorescence recovery after photobleaching, Cell biology, mCherry

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