2012AIP conference proceedingsRequires access

A Monte Carlo simulation of light scattering on biological suspensions with bigger optical depth

Dan Chicea

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Abstract

In biological suspensions light is scattered by the suspended cells. When the concentration of the scattering centers increases multiple scattering becomes dominant. A main challenge in modeling light diffusion is to find an analytical expression that describes the multiple light scattering in an accurate manner, including light scattering anisotropy. The Henyey-Greenstein phase function embedded in the RWMCS code previously written was used to describe single scattering on Red Blood Cells in suspension. The results of the simulation, containing multiple light scattering, are used to verify the predictions of two effective phase functions. Previous published results revealed a good agreement with experimental data in the small concentration range for scattering centers having a diameter of several microns. The new simulation expanded the investigation in the bigger optical depth targets and the results are presented in the extended paper.

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What this paper is about

In biological suspensions light is scattered by the suspended cells. When the concentration of the scattering centers increases multiple scattering becomes dominant. A main challenge in modeling light diffusion is to find an analytical expression that describes the multiple light scattering in an accurate manner, including light scattering anisotropy. The Henyey-Greenstein phase function embedded in the RWMCS code previously written was used to describe single scattering on Red Blood Cells in suspension. The results of the simulation, containing multiple light scattering, are used to verify the predictions of two effective phase functions. Previous published results revealed a good agreement with experimental data in the small concentration range for scattering centers having a diameter of several microns. The new simulation expanded the investigation in the bigger optical depth targets and the results are presented in the extended paper.

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Available abstract

In biological suspensions light is scattered by the suspended cells. When the concentration of the scattering centers increases multiple scattering becomes dominant. A main challenge in modeling light diffusion is to find an analytical expression that describes the multiple light scattering in an accurate manner, including light scattering anisotropy. The Henyey-Greenstein phase function embedded in the RWMCS code previously written was used to describe single scattering on Red Blood Cells in suspension. The results of the simulation, containing multiple light scattering, are used to verify the predictions of two effective phase functions. Previous published results revealed a good agreement with experimental data in the small concentration range for scattering centers having a diameter of several microns. The new simulation expanded the investigation in the bigger optical depth targets and the results are presented in the extended paper.

Key concepts: Scattering, Light scattering, Optics, Monte Carlo method, Phase function, Static light scattering, Diffusion, Physics

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