Isolation and characterization of mutants of Trichosporon adeninovorans resistant to 2‐deoxy‐D‐glucose
R. Büttner, A. Scheit, R. Bode, D. Birnbaum
Abstract
R. Büttner, A. Scheit, R. Bode, D. Birnbaum
Abstract
Abstract We isolated stable mutants from Trichosporon adeninovorans resistant to 2‐deoxy‐D‐glucose. All mutants were altered in the C‐catabolite repression and produced different amounts of glucoamylase and β‐glucosidase when grown on several carbon sources. In addition to the C‐catabolite derepression of these extracellular enzymes, a repression defect of isocitrate lyase was found.
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Abstract We isolated stable mutants from Trichosporon adeninovorans resistant to 2‐deoxy‐D‐glucose. All mutants were altered in the C‐catabolite repression and produced different amounts of glucoamylase and β‐glucosidase when grown on several carbon sources. In addition to the C‐catabolite derepression of these extracellular enzymes, a repression defect of isocitrate lyase was found.
Key concepts: Catabolite repression, Derepression, Fed-batch culture, Mutant, Biochemistry, Extracellular, Isocitrate lyase, Chemistry