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TSH and Dibutyryl-Cyclic-AMP Stimulation of Hormone Release from Rat Thyroid Glandsin Vitro

Teiichiro Tonoue, Winton Tong, Viktor Štolc

Open publisher page 33 citations

Abstract

The release of hormones from thyroid glands in vitro was observed by incubating the whole thyroid lobes taken from rats which had been injected 3 hr previously with 131I. The release of free 131I-T3 and T4 from 131I-thyroglobulin that occurred in the tissues during 2 hr incubations was measured by paper chromatographic analysis of the tissues and media. TSH (50–500 mU/ml) increased the free T3, T4 content of the incubated specimens by 2- to 4-fold. Cyclic-AMP, ATP and AMP were without effect, but dibutyryl-cyclic-AMP (3 mm) stimulated T3, T4 release in a TSH-like manner. Furthermore, incubation with TSH led to prompt increases in the cyclic-AMP content of the thyroid lobes. These findings provide another example of a TSH action which may be mediated by the adenyl cyclase-cyclic AMP system. (Endocrinology86: 271, 1970)

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The release of hormones from thyroid glands in vitro was observed by incubating the whole thyroid lobes taken from rats which had been injected 3 hr previously with 131I. The release of free 131I-T3 and T4 from 131I-thyroglobulin that occurred in the tissues during 2 hr incubations was measured by paper chromatographic analysis of the tissues and media. TSH (50–500 mU/ml) increased the free T3, T4 content of the incubated specimens by 2- to 4-fold. Cyclic-AMP, ATP and AMP were without effect, but dibutyryl-cyclic-AMP (3 mm) stimulated T3, T4 release in a TSH-like manner. Furthermore, incubation with TSH led to prompt increases in the cyclic-AMP content of the thyroid lobes. These findings provide another example of a TSH action which may be mediated by the adenyl cyclase-cyclic AMP system. (Endocrinology86: 271, 1970)

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Available abstract

The release of hormones from thyroid glands in vitro was observed by incubating the whole thyroid lobes taken from rats which had been injected 3 hr previously with 131I. The release of free 131I-T3 and T4 from 131I-thyroglobulin that occurred in the tissues during 2 hr incubations was measured by paper chromatographic analysis of the tissues and media. TSH (50–500 mU/ml) increased the free T3, T4 content of the incubated specimens by 2- to 4-fold. Cyclic-AMP, ATP and AMP were without effect, but dibutyryl-cyclic-AMP (3 mm) stimulated T3, T4 release in a TSH-like manner. Furthermore, incubation with TSH led to prompt increases in the cyclic-AMP content of the thyroid lobes. These findings provide another example of a TSH action which may be mediated by the adenyl cyclase-cyclic AMP system. (Endocrinology86: 271, 1970)

Key concepts: Internal medicine, Endocrinology, Stimulation, Incubation, Thyroid, In vitro, Hormone, Cyclase

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