1976•Canadian Journal of Genetics and CytologyRequires access

DIFFERENTIAL FLUORESCENCE OF SISTER CHROMATIDS WITH 4′-6-DIAMIDINO-2-PHENYLINDOLE

M.S. Lin, O.S. Alfi, George N. Donnell

Abstract

Differential fluorescence of sister chromatids and sister chromatid exchanges (SCE) in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with 4'-6-diamidino-2-phenylindole (DAPI). The DAPI fluorescence appears to be more stable than that of the dye 33258 Hoechst and may provide a more sensitive method for the detection of SCE.

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Key concepts: Sister chromatids, Biology, Fluorescence, Sister, Genetics, Differential (mechanical device), Chromosome, Optics