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1998•FEMS Microbiology LettersRequires access

Construction and analysis ofluxCDABE-based plasmid sensors for investigatingN-acyl homoserine lactone-mediated quorum sensing

Michael K. Winson, Simon Swift, Leigh Fish, John P. Throup, Frieda JÃ ̧rgensen, Siri Ram Chhabra, Barrie W. Bycroft, Paul M. Williams, Gordon S.A.B. Stewart

Open publisher page 593 citations

Abstract

Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.

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What this paper is about

Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.

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Available abstract

Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.

Key concepts: Quorum sensing, Bioreporter, Homoserine, Plasmid, Reporter gene, Biology, Gene, Microbiology

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Construction and analysis ofluxCDABE-based plasmid sensors for investigatingN-acyl homoserine lactone-mediated quorum sensing — Research Paper | ScholarLens