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2006•Cold Spring Harbor ProtocolsRequires access

DNA Preparation and Needle Loading for Gene Delivery by Microinjection

David Allen Dean

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Abstract

INTRODUCTIONFor microinjection into cells, high-quality plasmid DNA is purified using standard procedures. The resulting preparation is then delivered into the microinjection needle by either a back-filling or a forward-filling approach.

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INTRODUCTIONFor microinjection into cells, high-quality plasmid DNA is purified using standard procedures. The resulting preparation is then delivered into the microinjection needle by either a back-filling or a forward-filling approach.

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Available abstract

INTRODUCTIONFor microinjection into cells, high-quality plasmid DNA is purified using standard procedures. The resulting preparation is then delivered into the microinjection needle by either a back-filling or a forward-filling approach.

Key concepts: Microinjection, DNA, Gene, Gene delivery, Chemistry, Genetics, Biology, Molecular biology

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