Protective effects of Tea ployphenol injection on cerebral ischemia reperfusion injury in mice

[Objective] To observe the neuroprotective effect and therapeutic window of tea ployphenol(TP) injection on cerebral ischemia reperfusion injury in mice.[Methods] The cerebral ischemia reperfusion model in mice was made by bilateral ligation of common carotid arteries for 10 minutes and reperfusion for 10 minutes,repeating for three times.Mices were arranged in 9 groups which were sham operation group,ischemia control group,nimodipine group,3 TP groups treated with different levels of dose(50,100,200 mg/kg,i.v.0h before the first ligation),3 TP groups treated with different administrated time(0 h before first ligation and 0 h,30 min after last reperfusion,i.v.100 mg/kg).The content of water in the cerebric tissues were measured and the changes of urtralstructure of BBB was observed under electron microscope(EM).[Results]①The content of brain water in TP 50 mg/kg,100 mg/kg,0 h before first ligation and 0 h,30 min after last reperfusion groups(79.59±0.45)%,(79.18±0.45)%,(79.18±0.45)%,(78.95±0.79)%,(79.22±0.89)% were less than that in ischemia control group(80.21±0.40%),P0.01.② The observation under electron microscope demonstrated that the severity of ischemic damage in TP groups was lighter than that in ischemic control group.[Conclusions]The result indicated the neuroprotective effect of TP injection on cerebral ischemia reperfusion injury in mice.