1977•Analytical LettersRequires access

Use of Catecholoxygenase for Determination of Catechol

B. Zane Egan, D.W. Holladay

Abstract

A cell extract from Trichosporon cutaneum containing catechol 1,2-oxygenase catalyzes the oxidation of catechol to cis,cis-muconic acid. The absorbance of the cis,cis-muconic acid at 260 nm can be correlated with the initial catechol concentration in the range 5 × 10−6 M to 5 × 10−5 M in aqueous solutions. This enzymatic assay has been applied to the determination of catechol in aqueous samples derived from coal processing.

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Key concepts: Catechol, Muconic acid, Chemistry, Absorbance, Aqueous solution, Catechol oxidase, Enzyme, Chromatography