2008•Biotechnology & Biotechnological EquipmentOpen access

Qualitative Triplex PCR for the Detection of Genetically Modified Soybean and Maize

Zorica T. Nikolić, Dragana Milošević, Milka Vujaković, Dragana Marinković, Aranka Jevtić, Svetlana Balešević-Tubić

Abstract

A molecular screening method based on multiplex PCR that involves amplification of specific soybean or maize sequences from plant DNA (lectin or zein) and the amplification of 35S promoter and NOS terminator,for the detection of genetically modified soybean and maize was developed. The new method is proposed,for the simulicmeous cletcctimt of tree genetic elements in the.same run as reliable method for rapid detection of genetically, modified plants with sensitivity of 0.1%.

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Key concepts: Genetically modified organism, Genetically modified crops, Biology, Polymerase chain reaction, Biotechnology, Genetically modified maize, Gene, Genetics