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2003•Optics LettersRequires access

Phase retrieval for high-numerical-aperture optical systems

Bridget M. Hanser, Mats G. Gustafsson, David A. Agard, John W. Sedat

Open publisher page 93 citations

Abstract

We describe a phase retrieval approach for intensity point-spread functions of high-numerical-aperture optical systems such as light microscopes. The method calculates a generalized pupil function defined on a spherical shell, using measured images at several defocus levels. The resultant pupil functionsreproduce measured point-source images significantly better than does an ideal imaging model. Availability of pupil function information will facilitate new approaches to aberration correction in such systems.

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What this paper is about

We describe a phase retrieval approach for intensity point-spread functions of high-numerical-aperture optical systems such as light microscopes. The method calculates a generalized pupil function defined on a spherical shell, using measured images at several defocus levels. The resultant pupil functionsreproduce measured point-source images significantly better than does an ideal imaging model. Availability of pupil function information will facilitate new approaches to aberration correction in such systems.

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Available abstract

We describe a phase retrieval approach for intensity point-spread functions of high-numerical-aperture optical systems such as light microscopes. The method calculates a generalized pupil function defined on a spherical shell, using measured images at several defocus levels. The resultant pupil functionsreproduce measured point-source images significantly better than does an ideal imaging model. Availability of pupil function information will facilitate new approaches to aberration correction in such systems.

Key concepts: Pupil function, Optics, Phase retrieval, Exit pupil, Point spread function, Pupil, Entrance pupil, Aperture (computer memory)

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