1987•Journal of Pharmacy and PharmacologyRequires access
Comparative biodistribution of methotrexate and monoclonal antibody-methotrexate complexes in mice
M. V. Pimm, Jane E Caten, J. A. Clegg, E Jacobs, R W Baldwin
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Abstract
The biodistribution of radiolabelled methotrexate and immune complexes of methotrexate and a murine monoclonal anti-methotrexate antibody has been compared in mice. Complexes formed in-vitro with the antibody, but not with control immunoglobulin. The complexes were, characteristically, acid labile. In-vivo, blood levels, organ distribution and whole body catabolism of methotrexate in immune complexes were similar to those of free antibody, and markedly different from those of free drug. These findings suggest the feasibility of prolonging the survival of drugs and altering in-vivo distribution using complexes with monoclonal antibodies.