2012•Advanced materials researchOpen access

Apoptosis of Human Leukemia Cell Line K562 Cells Induced by Hydroxycamptothecine

Shu Li Shao, Wei Wei Zhang, Wei Zhao, Guang Wu

Abstract

To observe the effects of hydroxycamptothecine(HCPT) on the growth and apoptosis of gastric cancer cell line. The viability of human leukemia cell line K562 was determined by MTT, cell morphology was observed under AO staining and electron microscopy, the apoptosis was measured using flow cytometry analysis. The results indicated that the growth of human leukemia cell line K562 was significantly inhibited by HCPT, and the IC50 was 8.0µg/mL. Morphological variations of apoptosis were observed at 48 hours treated with HCPT by AO staining and apoptosis body was observed under electron microscope. The results indicated that HCPT can suppress the growth of human leukemia cell line K562 and induce apoptosis of the cells.

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Key concepts: Apoptosis, K562 cells, Flow cytometry, Leukemia, Cell culture, MTT assay, Staining, Cell