2008•Molecular Ecology ResourcesRequires access

New microsatellite loci isolated from the field cricket Gryllus bimaculatus characterized in two cricket species, Gryllus bimaculatus and Gryllus campestris

Amanda J. Bretman, Deborah A. Dawson, Gavin John Horsburgh, Tom Tregenza

Abstract

We have developed a new set of 27 polymorphic markers for each of two cricket species, Gryllus bimaculatus and Gryllus campestris. Initially, 14 published G. bimaculatus loci were tested in G. campestris; however, only five loci were polymorphic. Therefore, we isolated an additional 50 new microsatellite loci from G. bimaculatus and tested these in both species. In a minimum of 20 individuals, 27 of the new loci were polymorphic in G. bimaculatus and 25 in G. campestris.

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Key concepts: Gryllus bimaculatus, Biology, Field cricket, Cricket, Orthoptera, Microsatellite, Botany, Zoology