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Application of FT-ICR MS for the Study of Protein Complexes

Yaqin Liu, Yuanjiang Pan

Open publisher page 8 citations

Abstract

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) represents a powerful technique for analyzing biological samples due to the ultrahigh resolving power, high mass accuracy, and multiple-stage tandem mass spectrometry (MSn). With the advent of electrospray ionization (ESI), determinations of binding stoichiometry and binding sites for protein complexes are available. This review summarizes the recent FT-ICR MS applications in characterization of protein complexes, such as protein-peptide complexes, protein-protein complexes, and protein-nucleic acid complexes. Especially, combined with ECD and SWIFT techniques, FT-ICR MS has unique ion manipulation capabilities and plays a critical role in the analysis of protein complexes.

About this research paper

What this paper is about

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) represents a powerful technique for analyzing biological samples due to the ultrahigh resolving power, high mass accuracy, and multiple-stage tandem mass spectrometry (MSn). With the advent of electrospray ionization (ESI), determinations of binding stoichiometry and binding sites for protein complexes are available. This review summarizes the recent FT-ICR MS applications in characterization of protein complexes, such as protein-peptide complexes, protein-protein complexes, and protein-nucleic acid complexes. Especially, combined with ECD and SWIFT techniques, FT-ICR MS has unique ion manipulation capabilities and plays a critical role in the analysis of protein complexes.

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OpenAlex reports 8 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) represents a powerful technique for analyzing biological samples due to the ultrahigh resolving power, high mass accuracy, and multiple-stage tandem mass spectrometry (MSn). With the advent of electrospray ionization (ESI), determinations of binding stoichiometry and binding sites for protein complexes are available. This review summarizes the recent FT-ICR MS applications in characterization of protein complexes, such as protein-peptide complexes, protein-protein complexes, and protein-nucleic acid complexes. Especially, combined with ECD and SWIFT techniques, FT-ICR MS has unique ion manipulation capabilities and plays a critical role in the analysis of protein complexes.

Key concepts: Fourier transform ion cyclotron resonance, Electron-capture dissociation, Chemistry, Mass spectrometry, Electrospray ionization, Protein mass spectrometry, Top-down proteomics, Tandem mass spectrometry

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