Regeneration and micropropagation of lemon cultivars in vitro from nodal explants

The effect of sterilization agents and the composition of the nutrient medium on lemon explants regeneration, micropropagation and rooting was investigated. Nodal segments of eight year old lemon trees cv. Novoaphonsky, Beskolyuchy, Udarnik, Meyer lemon were taken as explants during spring. Veltolen 0.2%, 0.2% HgOCl and hypochlorite Ca 7% solutions were used as disinfectants at different exposures. The best results for surface sterilization was obtained when shoots were deeped in Veltolen solution by about 40 min. In this case 58.7% sterile explants was reseived. Several combinations of BAP with NAA were used for optimization of cell proliferation and micropropagation. Maximum effect at the stage of proliferation was obtained by adding to the MS basal medium 0.1 mg/l BAP and 0.5 mg/l NAA Cultural media consisted of MS minerals and vitamins with 1 mg/l BAP and 0.1 mg/l NAA was stimulated multiplication of regenerated plants. Rooting of regenerants was obtained on 1/2 MS supplemented with 0.5 mg/l NAA alone.