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2010•Methods in molecular biologyRequires access

Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Technology

Ryan D. Morin, Yongjun Zhao, Anna‐Liisa Prabhu, Noreen Dhalla, Helen McDonald, Pawan K. Pandoh, Angela Tam, Thomas Zeng, Martin Hirst, Marco A. Marra

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Abstract

MicroRNAs are key regulators of gene expression in diverse biological processes and their importance in embryonic stem cells is indisputable. New 'next-generation' technologies such as Illumina massively parallel sequencing offer vast improvements, in both scale and sensitivity, to microRNA profiling studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for analyzing the resultant sequence data for measuring microRNA abundance.

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MicroRNAs are key regulators of gene expression in diverse biological processes and their importance in embryonic stem cells is indisputable. New 'next-generation' technologies such as Illumina massively parallel sequencing offer vast improvements, in both scale and sensitivity, to microRNA profiling studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for analyzing the resultant sequence data for measuring microRNA abundance.

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Available abstract

MicroRNAs are key regulators of gene expression in diverse biological processes and their importance in embryonic stem cells is indisputable. New 'next-generation' technologies such as Illumina massively parallel sequencing offer vast improvements, in both scale and sensitivity, to microRNA profiling studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for analyzing the resultant sequence data for measuring microRNA abundance.

Key concepts: Massive parallel sequencing, microRNA, Illumina dye sequencing, Computational biology, DNA sequencing, Biology, Deep sequencing, Massively parallel

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