In vitro synthesis and secretion of ecdysteroids by Drosophila melanogaster ovaries
Elaine C. Rubenstein, Thomas J. Kelly, Margaret B. Schwartz, Charles W. Woods
Abstract
Elaine C. Rubenstein, Thomas J. Kelly, Margaret B. Schwartz, Charles W. Woods
Abstract
Abstract The ovaries of adult Drosophila melanogaster were shown to produce and secrete ecdysteroids in vitro by an ecdysteroid radioimmunoassay. The major secretory products were determined by HPLC/RIA analysis to be ecdysone, 20‐hydroxyecdysone, and a highly polar fraction. Based on competitive binding studies, the secreted ecdysteroids contain 2–3 times more 20‐hydroxyecdysone than ecdysone.
OpenAlex reports 51 citations for this work. Citation counts describe recorded attention and do not establish research quality.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
Abstract The ovaries of adult Drosophila melanogaster were shown to produce and secrete ecdysteroids in vitro by an ecdysteroid radioimmunoassay. The major secretory products were determined by HPLC/RIA analysis to be ecdysone, 20‐hydroxyecdysone, and a highly polar fraction. Based on competitive binding studies, the secreted ecdysteroids contain 2–3 times more 20‐hydroxyecdysone than ecdysone.
Key concepts: Ecdysteroid, Ecdysone, Secretion, Radioimmunoassay, Drosophila melanogaster, 20-Hydroxyecdysone, In vitro, Internal medicine