1982•Journal of Experimental ZoologyRequires access

In vitro synthesis and secretion of ecdysteroids by Drosophila melanogaster ovaries

Elaine C. Rubenstein, Thomas J. Kelly, Margaret B. Schwartz, Charles W. Woods

Open publisher page 51 citations

Abstract

Abstract The ovaries of adult Drosophila melanogaster were shown to produce and secrete ecdysteroids in vitro by an ecdysteroid radioimmunoassay. The major secretory products were determined by HPLC/RIA analysis to be ecdysone, 20‐hydroxyecdysone, and a highly polar fraction. Based on competitive binding studies, the secreted ecdysteroids contain 2–3 times more 20‐hydroxyecdysone than ecdysone.

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Abstract The ovaries of adult Drosophila melanogaster were shown to produce and secrete ecdysteroids in vitro by an ecdysteroid radioimmunoassay. The major secretory products were determined by HPLC/RIA analysis to be ecdysone, 20‐hydroxyecdysone, and a highly polar fraction. Based on competitive binding studies, the secreted ecdysteroids contain 2–3 times more 20‐hydroxyecdysone than ecdysone.

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Available abstract

Abstract The ovaries of adult Drosophila melanogaster were shown to produce and secrete ecdysteroids in vitro by an ecdysteroid radioimmunoassay. The major secretory products were determined by HPLC/RIA analysis to be ecdysone, 20‐hydroxyecdysone, and a highly polar fraction. Based on competitive binding studies, the secreted ecdysteroids contain 2–3 times more 20‐hydroxyecdysone than ecdysone.

Key concepts: Ecdysteroid, Ecdysone, Secretion, Radioimmunoassay, Drosophila melanogaster, 20-Hydroxyecdysone, In vitro, Internal medicine

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