2014•Asian Pacific Journal of Tropical DiseaseOpen access
Molecular identification of Leishmania species in Taybad district, Iran
Salehi Ghodratollah, Abdolmajid Fata, Mohaghegh Mohammad Ali, Mousavi Bazzaz Sayyed Mojtaba, Rafatpanah Hushang, Movahedi Abdolghayom
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Abstract
To identify Leishmania species in patients with cutaneous leishmaniasis in the city of Taybad in Razavi Khorasan Province from April 2012 to March 2013. Among 52 persons who referred to Health Center of Taybad with suspected skin lesions, stained slide smears of 35 patients showed positive result for Leishmania. Also polymerase chain reaction assay performed using specific kDNA primers. Data of patients were analyzed with SPSS. Of 35 positive smears for Leishmania, 21 (60%) belonged to males and 14 (40%) belonged to females. Polymerase chain reaction bands were observed in all 35 samples of which 31 (88.6%) samples showed Leishmania tropica and 4 (11.4%) showed Leishmania major. The highest infected age group was 11–20 years old. Both anthroponotic cutaneous leishmaniasis and zoonotic cutaneous leishmaniasis are present in Taybad. Leishmania tropica is the dominant causative species for anthroponotic cutaneous leishmaniasis. Further study is recommended to discover probable reservoir and vector for Leishmania major in Taybad.