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Yoghurt whey medium for food‐borne yeasts

Mohammed I. Yamani

Open publisher page 6 citations

Abstract

Summary A selective medium based on yoghurt whey was developed for the enumeration of yeasts in foods. Yoghurt whey agar (YWA) was prepared by mixing one part of a sterile agar solution (5%) with two parts of a sterile whey filtrate obtained from autoclaved (121°C, 20min) plain set yoghurt. the performance of YWA in the enumeration of yeasts was compared with that of plate count agar plus antibiotics (APCA) and acidified potato dextrose agar (PDA‐A) by examining food samples and broth cultures of known yeast isolates. APCA ranked first, confirming the superiority of antibiotic amended media over the acidified ones. the overall performance of YWA was comparable to that of PDA‐A.

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What this paper is about

Summary A selective medium based on yoghurt whey was developed for the enumeration of yeasts in foods. Yoghurt whey agar (YWA) was prepared by mixing one part of a sterile agar solution (5%) with two parts of a sterile whey filtrate obtained from autoclaved (121°C, 20min) plain set yoghurt. the performance of YWA in the enumeration of yeasts was compared with that of plate count agar plus antibiotics (APCA) and acidified potato dextrose agar (PDA‐A) by examining food samples and broth cultures of known yeast isolates. APCA ranked first, confirming the superiority of antibiotic amended media over the acidified ones. the overall performance of YWA was comparable to that of PDA‐A.

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Available abstract

Summary A selective medium based on yoghurt whey was developed for the enumeration of yeasts in foods. Yoghurt whey agar (YWA) was prepared by mixing one part of a sterile agar solution (5%) with two parts of a sterile whey filtrate obtained from autoclaved (121°C, 20min) plain set yoghurt. the performance of YWA in the enumeration of yeasts was compared with that of plate count agar plus antibiotics (APCA) and acidified potato dextrose agar (PDA‐A) by examining food samples and broth cultures of known yeast isolates. APCA ranked first, confirming the superiority of antibiotic amended media over the acidified ones. the overall performance of YWA was comparable to that of PDA‐A.

Key concepts: Agar, Enumeration, Food science, Yeast, Yeast extract, Potato dextrose agar, Agar plate, Chemistry

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